actr2  (Cell Signaling Technology Inc)

Journal: Brain Communications

Article Title: Mesenchymal stem cells reverse disease-specific abnormalities in nociceptive regions of the brain

doi: 10.1093/braincomms/fcaf494

Figure Lengend Snippet: Infused MSCs suppress an increase in AMPA-associated gene expressions selectively in the SI of the PNI model or the thalamus of the SCI model. ( A ) AKAP5, ( B ) ACTR2, ( C ) SORBS2. mRNA expression of the SI of the PNI model (−1), the SI of the SCI model (−2), the thalamus of the PNI model (−3) and the thalamus of the SCI model (−4), respectively. Data are presented as mean ± SEM. * P < 0.05. N = 6. Each data point represents the mean FC per animal. ACTR2 , actin-related protein 2; AKAP5 , A-kinase anchor protein 5; AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid; MSC, mesenchymal stem cells; PNI, peripheral nerve injury; SCI, spinal cord injury; SEM, standard error of the mean; SORBS2 , Arg-binding protein 2. One-way ANOVA, and the Tukey post hoc test was used for subgroup comparisons. Given the small size, we confirmed all results with the Kruskal–Wallis two-way analysis of variance followed by the Bonferroni post hoc test.

Article Snippet: TaqMan Universal Master Mix II with uracil- N glycosylase and TaqMan Gene Expression Assays for A-Kinase Anchor Protein 5 ( AKAP5; Rn01786021_m1) , actin-related protein 2 ( ACTR2; Rn01434079_m1), Arg-binding protein 2 ( SORBS2 ; Rn00587190_m1) and glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ; Rn01775763_g1) were purchased (Thermo Fisher Scientific Inc., Waltham, MA). qRT-PCR analysis was performed in triplicate using PRISM7500 with 7500 software v2.3 (Thermo Fisher Scientific Inc.).

Techniques: Expressing, Binding Assay

Journal: Acta Neuropathologica

Article Title: Enhanced microglial dynamics and a paucity of tau seeding in the amyloid plaque microenvironment contribute to cognitive resilience in Alzheimer’s disease

doi: 10.1007/s00401-024-02775-1

Figure Lengend Snippet: Microglial ARP2 and CAP1 levels are increased in the plaque microenvironment of AsymAD MFGs. ARP2 (green) ( a ) or CAP1 (green) ( f ) staining in the brain of AsymAD and AD cases. IBA1 (red) and NucBlue (blue) were used to identify microglia and cell nuclei/amyloid, respectively (P = Plaque Location). Green images indicate ARP2 ( a )- and CAP1 ( a , f )-positive staining. b , g . Quantification of overall ARP2 (b) or CAP1 (g) intensity within 50 μm of the core plaque . c , h Mean ARP2 (c) or CAP1 (h) intensity within the IBA1 + area. d , i ARP2 (d) or CAP1 ( i ) and IBA1 immunostaining in plaque-free areas e , j . Quantification of the mean ARP2 ( e ) or CAP1 ( j ) intensity per IBA1 cell in plaque-free areas. In b, c, g and h, n = 14–19 cases per group were analyzed. The data are shown as the mean ± SEM, and significance was determined by unpaired Student’s t test. In e and j, n = 13–19 cases per group were analyzed. The data are shown as the means ± SEMs; one-way ANOVA was used following Tukey’s multiple comparisons test

Article Snippet: Three days post transfection, ACTR2 RNA was analyzed by qRT-PCR using TaqMan probes (Assay ID: Hs00855199-g1, ThermoFisher).

Techniques: Staining, Immunostaining

Journal: Nature Communications

Article Title: Machine learning predictor PSPire screens for phase-separating proteins lacking intrinsically disordered regions

doi: 10.1038/s41467-024-46445-y

Figure Lengend Snippet: a Confocal images of GFP-ACTR2, GFP-H3C15, and GFP-HPRT1 overexpressing in HeLa cells. Condensates were indicated by white arrows. Scale bars, 5 µm. b Confocal images of GFP-ZNF738, GFP-GTF2A2, and GFP-CA5B expressing in HeLa cells. Scale bars, 5 µm. c Confocal images of endogenous PGM1, TXNL4B, SERPINB4, and VPS26B in HeLa cells. EDC4 (red) marks the P-body. Scale bars, 5 µm. d Immunostaining images of endogenous S100A7 and RAB31 in HeLa cells under stress conditions induced by sodium arsenite. G3BP1 (red) marks the stress granules. Scale bars, 5 µm. e Differential interference contrast (DIC) images demonstrate PS of PGM1, SERPINB4, RAB31, and S100A7 under specified conditions. Scale bars, 2 µm. The imaging was independently repeated 3 times with similar observations.

Article Snippet: The genes of full length ACTR2, H3C15, HPRT1, ZNF738, GTF2A2, CA5B, RAB31, S100A7, SERPINB4, and PGM1 were synthesized by AZENTA.

Techniques: Expressing, Immunostaining, Imaging